Overview Slides:
The Wisconsin Center of Excellence in Genomics Science – This short slide presentation provides a visualization of the overall idea of our CEGS.
GENECAPP – This short slide presentation provides a brief description of the GENECAPP procedure developed by our CEGS.
Tools:
These bioinformatics tools have been developed to facilitate and help in the analysis and interpretation of mass spectrometry data.
Protocols and Methodology:
In vitro Model for GENECAPP Technology
Protocols and Methodology
Below we describe step-by-step the protocols for an in vitro model of the GENECAPP strategy of sequence-specific capture of protein-DNA complexes for Mass Spectrometric Protein Identification.The method has been published as: Wu C-H, Chen S, Shortreed MR, Kreitinger GM, Yuan Y, et al. (2011) Sequence-Specific Capture of Protein-DNA Complexes for Mass Spectrometric Protein Identification. PLoS ONE 6(10): e26217.
- GENECAPP Materials
- References GENECAPP
- FAM-Labeled Protein-DNA Complex Preparation-GENECAPP
- Formaldehyde Cross-Linking and Quenching-GENECAPP
- Buffer Exchange and Exonuclease Digestion – GENECAPP
- In Situ Photolithographic Oligonucleotide Array Design and Synthesis-GENECAPP
- Sequence-Specific Capture-GENECAPP
- On-Chip Protease Digestion-GENECAPP
- Analysis by Mass Spectrometry-GENECAPP