While technologies already exist for the genome-wide analysis of gene transcription and DNA methylation, there is a desperate need for new technologies that enable the comprehensive parallel analysis of all protein-DNA interactions (including histones) without prior knowledge or assumptionsSmith, Shortreed, and Olivier, Analyst. 2011 Aug 7; 136(15):3060-5
The regulation of gene transcription is fundamental to the existence of complex multicellular organisms such as humans.Wu et al. PloS One. 2011; 6 (10) :e26217
The GENECAPP strategy employs sequence-specific hybridization capture of a specific DNA fragment to allow the isolation and subsequent characterization of all proteins bound to that region.Wu et al. PloS One. 2011; 6 (10) :e26217
The parallel identification of proteins bound at specific genomic loci begins with fragmentation of cross-linked chromatin from fixed cells or tissues.Wu et al. PloS One. 2011; 6 (10) :e26217
The goal of the Wisconsin Center of Excellence in Genomics Science is to invent novel technologies that will characterize DNA-protein interactions on a genome-wide scale.